Coding

Part:BBa_K2568007:Design

Designed by: Manman Sun   Group: iGEM18_Jiangnan   (2018-09-24)


Virus receptor TfR with Kozak sequence and His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 721
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added kozak sequence to enhance translation efficiency and protein expression,and added His-tag to help us conduct subsequent experimental testing.


Source

We extracted the total RNA of MDCK cells, used TAKARA reverse transcription kit to reverse transcribe RNA and obtained cDNA. Using cDNA as a template, we amplified TfR by PCR.

References